Project Title

Biomarkers in Oestrogen-Associated Thrombosis and Recurrent Miscarriage.

Project Summary

Pro- and anti-coagulants are naturally produced in the liver that functions to maintain haemostasis. A higher risk of venous thrombosis can result from deficiencies in key haemostatic proteins and an imbalance in haemostasis can be caused by:

• A decline in anticoagulant Protein S levels due to increased circulating oestrogen levels during pregnancy;
• Oral contraceptive use which down-regulate coagulation factors; or
• Oestrogen replacement therapy.

To date, molecular mechanisms of oestrogen-mediated acquired Protein S deficiency have been poorly understood, impinging on progress in the development of treatments for pregnancy-related thrombosis.

PBI’s research has discovered a novel involvement of microRNAs in coagulation pathways. This study aims to utilise this knowledge to map oestrogen/hormone-responsive microRNAs, and decipher the role(s) of microRNAs in the regulation of haemostasis and thrombotic disorders.

Blood Disorder

• DVT
• Spontaneous miscarriage
• Pregnancy-related thrombosis

Patient Recruitment Details

Patient recruitment status: Open

Patients participating in this project are required to fill in a short questionnaire regarding medical and pregnancy history, including details about previous miscarriages, hormone treatment / IVF and major surgery.

Patients are required to provide a 40mL blood sample, collected by a fully trained phlebotomist or medical practitioner. Genetic material (DNA and RNA) from the blood will be collected and tested as part of the study. The blood samples may also be used in future research investigating factor(s) causing clotting disorders as well as Protein S deficiency.

Some individuals may experience mild bruising around the site of the venepuncture – this generally resolves within 1-3 days. No other complications are anticipated.

Number of Patients

30 pregnant and 30 non-pregnant women, with or without, contraceptive use.

Coordinating Centre and Central Laboratory

• PBI Research Division
• Murdoch University